Application
Fig1: Western blot analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ikaros antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Daudi cells with Ikaros antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Ikaros [JB50-38] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLL associated antigen KW 6 antibody DNA-binding protein Ikaros antibody hIk 1 antibody Hs.54452 antibody IK1 antibody Ikaros (zinc finger protein) antibody IKAROS antibody IKAROS family zinc finger 1 (Ikaros) antibody Ikaros family zinc finger protein 1 antibody Ikzf1 antibody IKZF1_HUMAN antibody LYF1 antibody Lymphoid transcription factor LyF-1 antibody PRO0758 antibody Zinc finger protein subfamily 1A 1 (Ikaros) antibody Zinc finger protein subfamily 1A 1 antibody Zinc finger protein, subfamily 1A, member 1 antibody ZNFN1A1 antibody |
| Product description | Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLL associated antigen KW 6 antibody
DNA-binding protein Ikaros antibody
hIk 1 antibody
Hs.54452 antibody
IK1 antibody
Ikaros (zinc finger protein) antibody
IKAROS antibody
IKAROS family zinc finger 1 (Ikaros) antibody
Ikaros family zinc finger protein 1 antibody
Ikzf1 antibody
IKZF1_HUMAN antibody
LYF1 antibody
Lymphoid transcription factor LyF-1 antibody
PRO0758 antibody
Zinc finger protein subfamily 1A 1 (Ikaros) antibody
Zinc finger protein subfamily 1A 1 antibody
Zinc finger protein subfamily 1A member 1 antibody ZNFN1A1 antibody |
|---|---|
| Molecular Weight(MW) | 57/48 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13422 |
|---|
Application
Fig1: Western blot analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ikaros antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Daudi cells with Ikaros antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human tonsil tissue, human spleen tissue, mouse spleen tissue, human thymus tissue lysate. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
