Application
Fig1: Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.Application
Fig2: ICC staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | CRM1 [JB35-22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Chromosome region maintenance 1 protein homolog antibody CRM 1 antibody CRM1 homolog antibody DKFZp686B1823 antibody emb antibody Exp 1 antibody Exp1 antibody Exportin 1 antibody Exportin-1 antibody Exportin1 antibody XPO 1 antibody xpo1 antibody XPO1_HUMAN antibody |
| Product description | Protein transport across the nucleus is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin alpha1 and karyopherin beta1 subunits. p62 glycoprotein is a nucleoporin that is not only involved in the nuclear import of proteins, but also the export of nascent mRNA strands. NTF2 (nuclear transport factor 2) interacts with nucleoporin p62 as a homodimer composed of two monomers, and may be an obligate component of functional p62. CRM1 has been shown to be an export receptor for leucine-rich proteins that contain the nuclear export signal (NES). |
| Immunogen | Recombinant protein within C terminal human CRM1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Chromosome region maintenance 1 protein homolog antibody CRM 1 antibody CRM1 homolog antibody DKFZp686B1823 antibody emb antibody Exp 1 antibody Exp1 antibody Exportin 1 antibody Exportin-1 antibody Exportin1 antibody XPO 1 antibody xpo1 antibody XPO1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 123 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.
Application
Fig2: ICC staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | 293T, Hela, A431, LOVO, SiHa, K562, rat skeletal muscle tissue, human lung cancer tissue, human tonsil tissue, mouse skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
