Application
Fig1: Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.Application
Fig2: ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | PCK2 [JB52-39] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 4.1.1.32 antibody GTP mitochondrial precursor antibody HGNC:8725 antibody mitochondrial antibody Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody OTTHUMP00000164700 antibody PCK2 antibody PCKGM_HUMAN antibody PE antibody PEP carboxykinase antibody PEPCK antibody PEPCK deficiency mitochondrial antibody PEPCK M antibody PEPCK-M antibody PEPCK2 antibody Phosphoenolpyruvate carboxykinase [GTP] antibody Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody |
| Product description | Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EC 4.1.1.32 antibody GTP mitochondrial precursor antibody HGNC:8725 antibody mitochondrial antibody Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody OTTHUMP00000164700 antibody PCK2 antibody PCKGM_HUMAN antibody PE antibody PEP carboxykinase antibody PEPCK antibody PEPCK deficiency mitochondrial antibody PEPCK M antibody PEPCK-M antibody PEPCK2 antibody Phosphoenolpyruvate carboxykinase [GTP] antibody Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.
Application
Fig2: ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue, human liver tissue, human colon cancer tissue, mouse colon tissue, human kidney tissue, MCF-7, HepG2. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
