Application
Fig1: Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution. Positive control： Lane1: human liver Lane2: mouse liver Lane3: rat liver Lane4: rat liver, preincubated with the immunization protein.Application
Fig2: ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | SDHB |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CWS2 antibody DHSB_HUMAN antibody FLJ92337 antibody Ip antibody Iron sulfur subunit antibody Iron sulfur subunit of complex II antibody Iron-sulfur subunit of complex II antibody mitochondrial antibody PGL 4 antibody PGL4 antibody SDH 1 antibody SDH antibody SDH1 antibody SDH2 antibody SDH2, homolog of antibody SdhB antibody SDHIP antibody Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody Succinate dehydrogenase complex iron sulfur subunit B antibody Succinate dehydrogenase complex subunit B iron sulfur antibody Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody Succinate dehydrogenase iron sulfur protein antibody |
| Product description | In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma. |
| Immunogen | Recombinant protein within human SDHB aa 100-300. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CWS2 antibody
DHSB_HUMAN antibody
FLJ92337 antibody
Ip antibody
Iron sulfur subunit antibody
Iron sulfur subunit of complex II antibody
Iron-sulfur subunit of complex II antibody
mitochondrial antibody
PGL 4 antibody
PGL4 antibody
SDH 1 antibody
SDH antibody
SDH1 antibody
SDH2 antibody
SDH2 homolog of antibody SdhB antibody SDHIP antibody Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial antibody Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody Succinate Dehydrogenase 2 S. cerevisiae homolog of antibody Succinate dehydrogenase complex iron sulfur subunit B antibody Succinate dehydrogenase complex subunit B iron sulfur antibody Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody succinate dehydrogenase complex subunit B iron sulfur (Ip) antibody Succinate dehydrogenase iron sulfur protein antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution. Positive control： Lane1: human liver Lane2: mouse liver Lane3: rat liver Lane4: rat liver, preincubated with the immunization protein.
Application
Fig2: ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat liver tissue, human liver tissue, mouse liver tissue, 293T, HT-29, rat heart tissue, human kidney tissue, human skin tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
