Application
Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.Application
Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | ITPR2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4 antibody 5-trisphosphate receptor antibody Inositol 1 4 5 trisphosphate receptor type 2 antibody Inositol 1,4,5-trisphosphate receptor type 2 antibody Inositol 145 trisphosphate receptor type 2 antibody InsP3 R2 antibody InsP3R2 antibody IP3 R2 antibody IP3 receptor antibody IP3 receptor isoform 2 antibody IP3R 2 antibody IP3R2 antibody ITPR 2 antibody Itpr2 antibody ITPR2_HUMAN antibody Type 2 inositol 1 4 5 trisphosphate receptor antibody Type 2 inositol 1 antibody Type 2 inositol 145 trisphosphate receptor antibody Type 2 InsP3 receptor antibody |
| Product description | The protein encoded by this gene belongs to the inositol 1,4,5-triphosphate receptor family, whose members are second messenger intracellular calcium release channels. These proteins mediate a rise in cytoplasmic calcium in response to receptor activated production of inositol triphosphate. Inositol triphosphate receptor-mediated signaling is involved in many processes including cell migration, cell division, smooth muscle contraction, and neuronal signaling. This protein is a type 2 receptor that consists of a cytoplasmic amino-terminus that binds inositol triphosphate, six membrane-spanning helices that contribute to the ion pore, and a short cytoplasmic carboxy-terminus. A mutation in this gene has been associated with anhidrosis, suggesting that intracellular calcium release mediated by this protein is required for eccrine sweat production. |
| Immunogen | Recombinant protein within human ITPR2 aa 1100-1450. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICCIHCFC |
| Dot Blot:1:2,000-1:5,000 ICC:1:50-1:100 IHC:1:50 -1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 4 antibody
5-trisphosphate receptor antibody
Inositol 1 4 5 trisphosphate receptor type 2 antibody
Inositol 1 4 5-trisphosphate receptor type 2 antibody Inositol 145 trisphosphate receptor type 2 antibody InsP3 R2 antibody InsP3R2 antibody IP3 R2 antibody IP3 receptor antibody IP3 receptor isoform 2 antibody IP3R 2 antibody IP3R2 antibody ITPR 2 antibody Itpr2 antibody ITPR2_HUMAN antibody Type 2 inositol 1 4 5 trisphosphate receptor antibody Type 2 inositol 1 antibody Type 2 inositol 145 trisphosphate receptor antibody Type 2 InsP3 receptor antibody |
|---|---|
| Molecular Weight(MW) | 308 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. |
Application
Fig1: Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.
Application
Fig2: ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HepG2, HT-29, SiHa, rat kidney tissue, human colon tissue, human breast tissue, mouse liver tissue. |
|---|---|
| Application Notes | Dot Blot:1:2,000-1:5,000 ICC:1:50-1:100 IHC:1:50 -1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
