Application
Fig1: Western blot analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | NPHS2 [JB51-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nephrosis 2, idiopathic, steroid resistant (podocin) antibody nephrosis 2, idiopathic, steroid resistant antibody NPHS2 antibody NPHS2 gene antibody PDCN antibody PODO_HUMAN antibody Podocin antibody SRN1 antibody |
| Product description | Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Nephrosis 2 idiopathic steroid resistant (podocin) antibody nephrosis 2 idiopathic steroid resistant antibody NPHS2 antibody NPHS2 gene antibody PDCN antibody PODO_HUMAN antibody Podocin antibody SRN1 antibody |
|---|---|
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cell membrane. Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of NPHS2 on rat kidney (1) and human kidney (2) tissue lysate using anti-NPHS2 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NPHS2 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue, human kidney tissue, mouse kidney tissue, 293T. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
