Application
Fig1: ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.| Product Name | TGF alpha [JB58-36] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EGF like TGF antibody EGF-like TGF antibody ETGF antibody TFGA antibody TGF A antibody TGF type 1 antibody TGF-alpha antibody Tgfa antibody TGFA_HUMAN antibody Transforming growth factor alpha antibody Transforming growth factor alpha precursor antibody Wa1 antibody Waved 1 antibody |
| Product description | Transforming growth factor-α (TGFα) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFα is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFα binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-β. TGFα is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFα bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCIP |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EGF like TGF antibody EGF-like TGF antibody ETGF antibody TFGA antibody TGF A antibody TGF type 1 antibody TGF-alpha antibody Tgfa antibody TGFA_HUMAN antibody Transforming growth factor alpha antibody Transforming growth factor alpha precursor antibody Wa1 antibody Waved 1 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa |
| Cellular Localization | Plasma membrane. Extracellular region. Secreted. |
| SwissProt ID | P01135 |
|---|
Application
Fig1: ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining TGF alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TGF alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TGF alpha antibody. Counter stained with hematoxylin.| Positive Control | Human tonsil tissue, human liver tissue, human colon cancer tissue, human kidney tissue, A431, A549, PC-3M. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
