Application
Fig1: ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Apolipoprotein A II in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Apolipoprotein A II [JB70-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APO A2 antibody Apo AII antibody Apo-AII antibody APOA 2 antibody ApoA II antibody ApoA-II antibody APOA2 antibody APOA2_HUMAN antibody APOAII antibody Apolipoprotein A II antibody Apolipoprotein A-II(1-76) antibody Apolipoprotein A2 antibody Apolipoprotein AII antibody ApolipoproteinA II antibody OTTHUMP00000032244 antibody |
| Product description | s are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a co-factor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesterylesters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | APO A2 antibody Apo AII antibody Apo-AII antibody APOA 2 antibody ApoA II antibody ApoA-II antibody APOA2 antibody APOA2_HUMAN antibody APOAII antibody Apolipoprotein A II antibody Apolipoprotein A-II(1-76) antibody Apolipoprotein A2 antibody Apolipoprotein AII antibody ApolipoproteinA II antibody OTTHUMP00000032244 antibody |
|---|---|
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Apolipoprotein A II in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HepG2, LOVO, SH-SY-5Y, rat big intestine tissue, rat liver tissue, human liver tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
