Application
Fig1: Western blot analysis of CBARA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse kidney tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Hela cell lysate| Product Name | CBARA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Allergen Hom s 4 antibody ara CALC antibody atopy related autoantigen antibody atopy related autoantigen CALC antibody Atopy-related autoantigen CALC antibody CALC antibody calcium binding atopy related autoantigen 1 antibody Calcium uptake protein 1 antibody calcium uptake protein 1, mitochondrial antibody Calcium-binding atopy-related autoantigen 1 antibody CBARA1 antibody EFHA3 antibody FLJ12684 antibody MICU1 antibody MICU1_HUMAN antibody mitochondrial antibody mitochondrial calcium uptake 1 antibody MPXPS antibody |
| Product description | Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Acts as a gatekeeper of MCU at low concentration of calcium, preventing channel opening. Enhances MCU opening at high calcium concentration, allowing a rapid response of mitochondria to calcium signals generated in the cytoplasm. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Immunogen | Recombinant protein within Human CBARA1 aa 14-154. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Allergen Hom s 4 antibody
ara CALC antibody
atopy related autoantigen antibody
atopy related autoantigen CALC antibody
Atopy-related autoantigen CALC antibody
CALC antibody
calcium binding atopy related autoantigen 1 antibody
Calcium uptake protein 1 antibody
calcium uptake protein 1 mitochondrial antibody Calcium-binding atopy-related autoantigen 1 antibody CBARA1 antibody EFHA3 antibody FLJ12684 antibody MICU1 antibody MICU1_HUMAN antibody mitochondrial antibody mitochondrial calcium uptake 1 antibody MPXPS antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of CBARA1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse kidney tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Hela cell lysate| Positive Control | Mouse kidney tissue, rat brain tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
