Application
Fig1: Western blot analysis of STIP1 on Mouse testis and Rat testis lysates using anti-STIP1 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STIP1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STIP1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STIP1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of K562 cells with STIP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | STIP1 [JB95-38] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Epididymis secretory sperm binding protein Li 94n antibody HEL S 94n antibody Hop antibody Hsc70/Hsp90 organizing protein antibody Hsc70/Hsp90-organizing protein antibody IEF SSP 3521 antibody NY REN 11 antigen antibody P60 antibody Renal carcinoma antigen NY-REN-11 antibody STI1 antibody STI1L antibody STIP1 antibody STIP1_HUMAN antibody Stress induced phosphoprotein 1 antibody Stress-induced-phosphoprotein 1 antibody Transformation sensitive protein IEF SSP 3521 antibody Transformation-sensitive protein IEF SSP 3521 antibody |
| Product description | Stress-induced-phosphoprotein 1 (STI1) functions as a co-chaperone for HSP70 and HSP90 during heat shock response. STI1 exists as either a monomer or a dimer, and this conformational flexibility facilitates its function in organizing HSP70/HSP90. HSP90 acts as an ATPase, and requires the recruitment of client proteins and proper conformation to function. STI1 acts as a "scaffold" for client protein recruitment to the relaxed, ADP-bound conformation of HSP90, thus suppressing ATP turnover during the loading phase and allowing proper function. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCFCIPICC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Epididymis secretory sperm binding protein Li 94n antibody HEL S 94n antibody Hop antibody Hsc70/Hsp90 organizing protein antibody Hsc70/Hsp90-organizing protein antibody IEF SSP 3521 antibody NY REN 11 antigen antibody P60 antibody Renal carcinoma antigen NY-REN-11 antibody STI1 antibody STI1L antibody STIP1 antibody STIP1_HUMAN antibody Stress induced phosphoprotein 1 antibody Stress-induced-phosphoprotein 1 antibody Transformation sensitive protein IEF SSP 3521 antibody Transformation-sensitive protein IEF SSP 3521 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of STIP1 on Mouse testis and Rat testis lysates using anti-STIP1 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STIP1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of K562 cells with STIP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse testis tissue, rat testis tissue lysate, human colon cancer tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 ICC:1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
