Application
Fig1: Western blot analysis of FKBP51 on rat kidney tissue and Daudi cell lysate using anti-FKBP51 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of Daudi cells with FKBP51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | FKBP51 [JB95-39] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 51 kDa FK506 binding protein 5 antibody 51 kDa FK506 binding protein antibody 51 kDa FK506-binding protein antibody 51 kDa FKBP antibody 54 kDa progesterone receptor associated immunophilin antibody 54 kDa progesterone receptor-associated immunophilin antibody AIG 6 antibody AIG6 antibody Androgen regulated protein 6 antibody Androgen-regulated protein 6 antibody FF1 antigen antibody FK506 binding protein 5 antibody FK506-binding protein 5 antibody FKBP 5 antibody FKBP 51 antibody FKBP 54 antibody FKBP-5 antibody FKBP-51 antibody FKBP5 antibody FKBP5_HUMAN antibody FKBP54 antibody HSP90 binding immunophilin antibody HSP90-binding immunophilin antibody MGC111006 antibody OTTHUMP00000016268 antibody P54 antibody Peptidyl prolyl cis trans isomerase antibody Peptidyl-prolyl cis-trans isomerase FKBP5 antibody Peptidylprolyl cis trans isomerase antibody PPIase antibody PPIase FKBP5 antibody Ptg 10 antibody Ptg10 antibody Rotamase antibody T cell FK506 binding protein antibody |
| Product description | The immunophilins are a highly conserved family of cis-trans peptidyl-prolyl isomerases that bind to and mediate the effects of immunosuppressive drugs, such as cyclosporin, FK506 and rapamycin. Several related immunophilins, FKBP12, FKBP51 and FKBP52, are characterized as cytosolic FK506-binding proteins, and following ligand binding, they functionally inhibit the phosphatase activity of calcineurin. The ubiquitously expressed FKBP12 also associates with the cytoplasmic domain of the TGFβ-type I receptor, where it stabilizes the inactive conformation of the receptor and blocks the activation of the TGFβ pathway. FKBP51 and FKBP52 are two highly related proteins. FKBP51 is predominantly expressed in T cells and is induced by glucocorticoids. FKBP51 mediates the effects of FK506 and rapamycin by inhibiting intracellular calcineurin activity, and by blocking T-cell activation and proliferation. FKBP52, known also as FKBP-59 or heat shock protein 56, is expressed in a variety of tissues and can also associate with the heat shock protein (hsp90) in mature steroid receptor complexes (6,8). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | 51 kDa FK506 binding protein 5 antibody 51 kDa FK506 binding protein antibody 51 kDa FK506-binding protein antibody 51 kDa FKBP antibody 54 kDa progesterone receptor associated immunophilin antibody 54 kDa progesterone receptor-associated immunophilin antibody AIG 6 antibody AIG6 antibody Androgen regulated protein 6 antibody Androgen-regulated protein 6 antibody FF1 antigen antibody FK506 binding protein 5 antibody FK506-binding protein 5 antibody FKBP 5 antibody FKBP 51 antibody FKBP 54 antibody FKBP-5 antibody FKBP-51 antibody FKBP5 antibody FKBP5_HUMAN antibody FKBP54 antibody HSP90 binding immunophilin antibody HSP90-binding immunophilin antibody MGC111006 antibody OTTHUMP00000016268 antibody P54 antibody Peptidyl prolyl cis trans isomerase antibody Peptidyl-prolyl cis-trans isomerase FKBP5 antibody Peptidylprolyl cis trans isomerase antibody PPIase antibody PPIase FKBP5 antibody Ptg 10 antibody Ptg10 antibody Rotamase antibody T cell FK506 binding protein antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | Q13451 |
|---|
Application
Fig1: Western blot analysis of FKBP51 on rat kidney tissue and Daudi cell lysate using anti-FKBP51 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue tissue using anti-FKBP51 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of Daudi cells with FKBP51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Rat kidney tissue and Daudi cell lysate, Rat lung tissue, human tonsil tissue, human kidney tissue, Daudi. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
