Application
Fig1: Western blot analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell (2) and Hela (3)cell lysates using anti-MCM3 antibody at 1/500 dilution.Application
Fig2: ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MCM3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | MCM3 [JB99-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cervical cancer proto oncogene 5 antibody DNA polymerase alpha holoenzyme associated P1 antibody DNA polymerase alpha holoenzyme associated protein P1 antibody DNA polymerase alpha holoenzyme-associated protein P1 antibody DNA replication factor MCM3 antibody DNA replication licensing factor mcm3 antibody HCC 5 antibody HCC5 antibody hRlf beta subunit antibody Human cervical cancer proto oncogene 5 antibody MCM 3 antibody mcm3 antibody MCM3 minichromosome maintenance deficient 3 antibody MCM3_HUMAN antibody MGC1157 antibody Minichromosome maintenance complex component 3 antibody Minichromosome maintenance deficient 3 antibody Minichromosome maintenance protein 3 antibody P1 h antibody P1 MCM3 antibody P1 Protein antibody P1-MCM3 antibody P1.h antibody p102 antibody P102 protein antibody Replication licensing factor beta subunit antibody RLF beta subunit antibody RLF subunit beta antibody RLFB antibody |
| Product description | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Cervical cancer proto oncogene 5 antibody DNA polymerase alpha holoenzyme associated P1 antibody DNA polymerase alpha holoenzyme associated protein P1 antibody DNA polymerase alpha holoenzyme-associated protein P1 antibody DNA replication factor MCM3 antibody DNA replication licensing factor mcm3 antibody HCC 5 antibody HCC5 antibody hRlf beta subunit antibody Human cervical cancer proto oncogene 5 antibody MCM 3 antibody mcm3 antibody MCM3 minichromosome maintenance deficient 3 antibody MCM3_HUMAN antibody MGC1157 antibody Minichromosome maintenance complex component 3 antibody Minichromosome maintenance deficient 3 antibody Minichromosome maintenance protein 3 antibody P1 h antibody P1 MCM3 antibody P1 Protein antibody P1-MCM3 antibody P1.h antibody p102 antibody P102 protein antibody Replication licensing factor beta subunit antibody RLF beta subunit antibody RLF subunit beta antibody RLFB antibody |
|---|---|
| Molecular Weight(MW) | 102 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell (2) and Hela (3)cell lysates using anti-MCM3 antibody at 1/500 dilution.
Application
Fig2: ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse thymus tissue lysate, NIH-3T3, Hela, 293T, HepG2, SH-SY-5Y, rat testis tissue, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
