Application
Fig1: Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin.| Product Name | Rice Os01g0847700 protein |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Has oxidoreductase activity. |
| Immunogen | Synthetic peptide within Rice Os01g0847700 aa 292-316. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:500-1:1000: IF:1:50-1:200: IHC:1:50-1:200: |
|
| Species Reactivity | Rice |
| Concentration | 1mg/ml |
| Molecular Weight(MW) | 36 kDa |
|---|---|
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q0JHR5 |
|---|
Application
Fig1: Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin.| Positive Control | Rice stem. |
|---|---|
| Application Notes | WB:1:500-1:1000: IF:1:50-1:200: IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
