Application
Fig1: Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse skeletal muscle Lane 3: Mouse cerebellum Lane 4: Rat skin Lane 5: DaudiApplication
Fig2: ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | eEF1A1 [JB44-13] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCS 3 antibody CCS3 antibody Cervical cancer suppressor 3 antibody chunp6927 antibody CTCL tumor antigen antibody EE1A1 antibody EEF 1 antibody EEF1A antibody eEF1A-1 antibody EEF1A1 antibody EF-1-alpha-1 antibody EF-Tu antibody EF1A antibody EF1a like protein antibody EF1A1_HUMAN antibody Elongation factor 1 alpha subunit antibody Elongation factor 1-alpha 1 antibody Elongation factor Tu antibody Eukaryotic elongation factor 1 A-1 antibody Eukaryotic translation elongation factor 1 alpha 1 antibody Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody Glucocorticoid receptor AF 1 specific elongation factor antibody GRAF 1EF antibody HNGC:16303 antibody ik:tdsubc_2a3 antibody ik:tdsubc_2b3 antibody LENG7 antibody Leukocyte receptor cluster (LRC) member 7 antibody Leukocyte receptor cluster member 7 antibody Prostate tumor inducing protein 1 antibody PTI1 antibody tdsubc_2a3 antibody Translation elongation factor 1 alpha 1 like 14 antibody wu:fa91c07 antibody wu:fa94b03 antibody wu:fi13b09 antibody xx:tdsubc_2a3 antibody xx:tdsubc_2b3 antibody |
| Product description | The elongation factor-1 complex is composed of two subunits, EF-1 α1 (elongation factor 1-alpha 1) and EF-1 α2 (elongation factor 1-alpha 2), and is responsible for the delivery of aminoacyl tRNAs to the ribosome. EF-1 α1 is expressed predominately in brain, placenta, lung, liver, kidney and pancreas, while EF-1 α2 is highly expressed in heart, brain and skeletal muscle. Both EF-1 α1 and α2 localize to the nucleus and belong to the GTP-binding elongation factor family. The gene encoding EF-1 α2, which maps to human chromosome 20q13.3, may play a role in the development of ovarian cancer, while the EF-1 α1 gene, mapping to chromosome 6Q14.1, is commonly present as an autoantigen in patients with Felty syndrome. Felty syndrome is a disorder characterized by rheumatoid arthritis, a swollen spleen, decreased white blood cell count, and increased susceptibility to infection. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CCS 3 antibody CCS3 antibody Cervical cancer suppressor 3 antibody chunp6927 antibody CTCL tumor antigen antibody EE1A1 antibody EEF 1 antibody EEF1A antibody eEF1A-1 antibody EEF1A1 antibody EF-1-alpha-1 antibody EF-Tu antibody EF1A antibody EF1a like protein antibody EF1A1_HUMAN antibody Elongation factor 1 alpha subunit antibody Elongation factor 1-alpha 1 antibody Elongation factor Tu antibody Eukaryotic elongation factor 1 A-1 antibody Eukaryotic translation elongation factor 1 alpha 1 antibody Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody Glucocorticoid receptor AF 1 specific elongation factor antibody GRAF 1EF antibody HNGC:16303 antibody ik:tdsubc_2a3 antibody ik:tdsubc_2b3 antibody LENG7 antibody Leukocyte receptor cluster (LRC) member 7 antibody Leukocyte receptor cluster member 7 antibody Prostate tumor inducing protein 1 antibody PTI1 antibody tdsubc_2a3 antibody Translation elongation factor 1 alpha 1 like 14 antibody wu:fa91c07 antibody wu:fa94b03 antibody wu:fi13b09 antibody xx:tdsubc_2a3 antibody xx:tdsubc_2b3 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm. Membrane. Nucleus. |
Application
Fig1: Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse skeletal muscle Lane 3: Mouse cerebellum Lane 4: Rat skin Lane 5: Daudi
Application
Fig2: ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat brain tissue, mouse skeletal muscle tissue lysate, rat skin tissue lysate, Daudi, mouse cerebellum tissue lysate, HepG2, HUVEC, SH-SY-5Y, rat skeletal muscle tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse smooth muscle tissue, THP-1. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
