Application
Fig1: Western blot analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution.Application
Fig2: ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MBD3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MBD3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MBD3 antibody. Counter stained with hematoxylin.Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with MBD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | MBD3 [JB61-47] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI181826 antibody AU019209 antibody MBD 3 antibody Mbd3 antibody MBD3: methyl CpG binding domain protein 3 antibody MBD3_HUMAN antibody Methyl CpG binding domain protein 3 antibody Methyl CpG binding protein MBD3 antibody Methyl-CpG-binding domain protein 3 antibody Methyl-CpG-binding protein MBD3 antibody |
| Product description | Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5′ cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AI181826 antibody AU019209 antibody MBD 3 antibody Mbd3 antibody MBD3: methyl CpG binding domain protein 3 antibody MBD3_HUMAN antibody Methyl CpG binding domain protein 3 antibody Methyl CpG binding protein MBD3 antibody Methyl-CpG-binding domain protein 3 antibody Methyl-CpG-binding protein MBD3 antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of MBD3 on U937 cell lysate using anti-MBD3 antibody at 1/500 dilution.
Application
Fig2: ICC staining MBD3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MBD3 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MBD3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-MBD3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MBD3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MBD3 antibody. Counter stained with hematoxylin.
Application
Fig8: Flow cytometric analysis of SH-SY-5Y cells with MBD3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | U937, A549, NIH-3T3, SH-SY-5Y, rat testis tissue, human placenta tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
