Application
Fig1: Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Flow cytometric analysis of NPY2R / Y2 receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | NPY2R / Y2 receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuropeptide Y receptor type 2 antibody Neuropeptide Y receptor Y2 antibody NPY-Y2 receptor antibody NPY2-R antibody NPY2R antibody NPY2R_HUMAN antibody Y2 receptor antibody |
| Product description | Neuropeptide Y (NPY) and its family of five receptors are known to be associated with hypothalamic regulation of feeding behavior, metabolism, and energy homeostasis in both rodents and humans. In particular, knock-out mice for the gene neuropeptide Y 2 receptor, NPY2R, exhibit hyperphagia and excessive weight gain. As a result, research on the gene has focused on genotyping analysis for the identification of single nucleotide polymorphisms (SNPs) in obese human subjects and drug development for manipulation of its expression as a novel therapeutic treatment for obesity. Although primarily expressed in the arcuate nucleus of the hypothalamus, NPY2R may also be found in other tissues including trabecular bone, vascular tissue, and colonic mucosa. |
| Immunogen | Synthetic peptide within Mouse NPY2R / Y2 receptor aa 341-369. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-1:1000: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Neuropeptide Y receptor type 2 antibody
Neuropeptide Y receptor Y2 antibody
NPY-Y2 receptor antibody NPY2-R antibody NPY2R antibody NPY2R_HUMAN antibody Y2 receptor antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa (Predicted band size) |
| Cellular Localization | Membrane. |
| SwissProt ID | P49146 |
|---|
Application
Fig1: Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Flow cytometric analysis of NPY2R / Y2 receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | MCF-7 cell lysates. |
|---|---|
| Application Notes | WB:1:500-1:1000: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
