Application
Fig1: Western blot analysis of B3GAT1 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-15, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-15, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Flow cytometric analysis of B3GAT1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | B3GAT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3-glucuronyltransferase 1 antibody 3-glucuronyltransferase antibody B3GA1_HUMAN antibody B3gat1 antibody Beta 1 3 glucuronyltransferase 1 antibody Beta-1 antibody Beta-1,3-glucuronyltransferase 1 antibody CD 57 antibody CD57 antibody CD57 antigen antibody Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody GlcAT P antibody GlcAT-P antibody GLCATP antibody GlcUAT P antibody GlcUAT-P antibody GlcUATP antibody Glucuronosyltransferase P antibody HNK 1 antibody HNK1 antibody LEU 7 antibody LEU7 antibody LEU7 antigen antibody NK 1 antibody NK1 antibody UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody UDP-GlcUA:glycoprotein beta-1 antibody |
| Product description | Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1) is an enzyme that in humans is encoded by the B3GAT1 gene, whose enzymatic activity creates the CD57 epitope on other cell surface proteins. In immunology, the CD57 antigen (CD stands for cluster of differentiation) is also known as HNK1 (human natural killer-1) or LEU7. It is expressed as a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. Neoplastic CD57 positive cells are seen in conditions as varied as large granular lymphocytic leukaemia, small-cell carcinoma, thyroid carcinoma, and neural and carcinoid tumours. Although the antigen is particularly common in carcinoid tumours, it is found in such a wide range of other conditions that it is of less use in distinguishing these tumours from others than more specific markers such as chromogranin and NSE. |
| Immunogen | Synthetic peptide within Human B3GAT1 aa 30-60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-1:1000: IHC-P:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 3-glucuronyltransferase 1 antibody
3-glucuronyltransferase antibody
B3GA1_HUMAN antibody B3gat1 antibody Beta 1 3 glucuronyltransferase 1 antibody Beta-1 antibody Beta-1 3-glucuronyltransferase 1 antibody CD 57 antibody CD57 antibody CD57 antigen antibody Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody GlcAT P antibody GlcAT-P antibody GLCATP antibody GlcUAT P antibody GlcUAT-P antibody GlcUATP antibody Glucuronosyltransferase P antibody HNK 1 antibody HNK1 antibody LEU 7 antibody LEU7 antibody LEU7 antigen antibody NK 1 antibody NK1 antibody UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody UDP-GlcUA:glycoprotein beta-1 antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Golgi apparatus. Secreted. Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of B3GAT1 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-15, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-15, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Flow cytometric analysis of B3GAT1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Rat brain tissue, mouse brain tissue, SH-SY5Y. |
|---|---|
| Application Notes | WB:1:500-1:1000: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
