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SNF5 [JB49-18]
Catalog Number:OM629199
OM629199
Discount Price
OM629199-100ul
1~2 Week
100ul
OM629199-50ul
50ul
OM629199-20ul
20ul
1~2 Week
Product Profile
Product Name SNF5 [JB49-18]
Antibody Type Primary Antibodies
Antigen Alias BAF47 antibody BRG1-associated factor 47 antibody hSNF5 antibody INI1 antibody Integrase interactor 1 protein antibody Malignant rhabdoid tumor suppressor antibody RDT antibody RTPS1 antibody Sfh1p antibody SMARCB1 antibody SNF5 homolog antibody SNF5_HUMAN antibody SNF5L1 antibody Snr1 antibody Sucrose nonfermenting yeast homolog like 1 antibody SWI/SNF complex component SNF5 antibody SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody SWI10 antibody Transcription factor TYE4 antibody Transcription regulatory protein SNF5 antibody TYE4 antibody
Product description The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
Immunogen Synthetic peptide of C terminal Human SNF5.
Key Feature
Clonality Monoclonal
Isotype IgG
Host Species Recombinant rabbit
Tested Applications WBIHCFC
WB:1:500-1:1,000
IHC:1:50-1:100
FC:1:50-1:100
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Target Information
Alternative Names BAF47 antibody BRG1-associated factor 47 antibody hSNF5 antibody INI1 antibody Integrase interactor 1 protein antibody Malignant rhabdoid tumor suppressor antibody RDT antibody RTPS1 antibody Sfh1p antibody SMARCB1 antibody SNF5 homolog antibody SNF5_HUMAN antibody SNF5L1 antibody Snr1 antibody Sucrose nonfermenting yeast homolog like 1 antibody SWI/SNF complex component SNF5 antibody SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody SWI10 antibody Transcription factor TYE4 antibody Transcription regulatory protein SNF5 antibody TYE4 antibody
Molecular Weight(MW) 44 kDa
Cellular Localization Nucleus.
Database Links
SwissProt ID Q12824 Q9Z0H3
Application

Application

Fig1: Western blot analysis of SNF5 on Daudi cell and mouse lymph node tissue lysates using anti-SNF5 antibody at 1/500 dilution.

Application

Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SNF5 antibody. Counter stained with hematoxylin.

Application

Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SNF5 antibody. Counter stained with hematoxylin.

Application

Fig4: Flow cytometric analysis of SH-SY-5Y cells with SNF5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Positive Control Daudi, mouse lymph node tissue lysate, rat kidney tissue, mouse colon tissue, SH-SY-5Y.
Application Notes WB:1:500-1:1,000
IHC:1:50-1:100
FC:1:50-1:100
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
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