Application
Fig1: Western blot analysis of CD146 on SiHa cell using anti-CD146 antibody at 1/500 dilution.Application
Fig2: ICC staining CD146 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD146 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD146 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HUVEC cells with CD146 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD146 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors. The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease. Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin. Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function. |
| Immunogen | Synthetic peptide within C-terminal human CD146 (Cytoplasmic). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | FCICCIFIHCWB |
| WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | A32 antigen antibody CD 146 antibody CD146 antibody CD146 antigen antibody Cell surface glycoprotein MUC18 antibody Cell surface glycoprotein P1H12 antibody Gicerin antibody Mcam antibody Melanoma adhesion molecule antibody Melanoma associated antigen A32 antibody Melanoma associated antigen MUC18 antibody Melanoma associated glycoprotein MUC18 antibody Melanoma cell adhesion molecule antibody Melanoma-associated antigen A32 antibody Melanoma-associated antigen MUC18 antibody MelCAM antibody MUC 18 antibody MUC18 antibody MUC18_HUMAN antibody S endo 1 antibody S endo 1 endothelial associated antigen antibody S-endo 1 endothelial-associated antigen antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of CD146 on SiHa cell using anti-CD146 antibody at 1/500 dilution.
Application
Fig2: ICC staining CD146 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD146 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD146 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HUVEC cells with CD146 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, Hela, HUVEC, rat brain tissue, human tonsil tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
