Application
Fig1: ICC staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining Phospho-Creb (S133) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HUVEC cells with Phospho-Creb (S133) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Phospho-Creb (S133) [JB25-40] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Active transcription factor CREB antibody cAMP response element binding protein 1 antibody cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody |
| Product description | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb. |
| Modification | p-S133 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIFIHCIPFC |
| ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Active transcription factor CREB antibody
cAMP response element binding protein 1 antibody
cAMP response element binding protein antibody cAMP responsive element binding protein 1 antibody cAMP-responsive element-binding protein 1 antibody CREB antibody CREB-1 antibody CREB1 antibody CREB1_HUMAN antibody Cyclic AMP-responsive element-binding protein 1 antibody MGC9284 antibody OTTHUMP00000163864 antibody OTTHUMP00000163865 antibody OTTHUMP00000206660 antibody OTTHUMP00000206662 antibody OTTHUMP00000206667 antibody Transactivator protein antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 37 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: ICC staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining Phospho-Creb (S133) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HUVEC cells with Phospho-Creb (S133) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Human colon tissue lysates, HUVEC, SH-SY-5Y, human colon cancer tissue, human spleen tissue, mouse colon tissue. |
|---|---|
| Application Notes | ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
