Application
Fig1: Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.Application
Fig2: ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Glucose 6 phosphate isomerase [JG33-86] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMF antibody Aurocrine motility factor antibody Autocrine motility factor antibody DKFZp686C13233 antibody EC 5.3.1.9 antibody G6PI_HUMAN antibody Glucose phosphate isomerase antibody Glucose-6-phosphate isomerase antibody GNPI antibody GPI antibody Gpi1 antibody Hexose monophosphate isomerase antibody Hexosephosphate isomerase antibody Neuroleukin antibody NLK antibody Oxoisomerase antibody PGI antibody PHI antibody Phosphoglucose isomerase antibody Phosphohexomutase antibody Phosphohexose isomerase antibody Phosphosaccharomutase antibody SA 36 antibody SA-36 antibody SA36 antibody |
| Product description | Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment. |
| Immunogen | Recombinant protein corresponding to human Glucose 6 phosphate isomerase 300-500aa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AMF antibody Aurocrine motility factor antibody Autocrine motility factor antibody DKFZp686C13233 antibody EC 5.3.1.9 antibody G6PI_HUMAN antibody Glucose phosphate isomerase antibody Glucose-6-phosphate isomerase antibody GNPI antibody GPI antibody Gpi1 antibody Hexose monophosphate isomerase antibody Hexosephosphate isomerase antibody Neuroleukin antibody NLK antibody Oxoisomerase antibody PGI antibody PHI antibody Phosphoglucose isomerase antibody Phosphohexomutase antibody Phosphohexose isomerase antibody Phosphosaccharomutase antibody SA 36 antibody SA-36 antibody SA36 antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Cytoplasm. Secreted. |
| SwissProt ID | P06744 |
|---|
Application
Fig1: Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, HepG2, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
