Application
Fig1: Western blot analysis of ATG4A on rat bone marrow. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ATG4A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI627006 antibody APG4 autophagy 4 homolog A antibody Apg4a antibody ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody ATG4 autophagy related 4 homolog A antibody ATG4A antibody ATG4A_HUMAN antibody Atg4al antibody AUT like 2 cysteine endopeptidase antibody AUT-like 2 cysteine endopeptidase antibody Autl2 antibody Autophagin 2 antibody Autophagin-2 antibody Autophagy related 4A cysteine peptidase antibody Autophagy related cysteine endopeptidase 2 antibody Autophagy related protein 4 homolog A antibody Autophagy-related cysteine endopeptidase 2 antibody Autophagy-related protein 4 homolog A antibody AV169859 antibody Cysteine protease ATG4A antibody hAPG4A antibody MGC107179 antibody |
| Product description | Cysteine protease ATG4A is an enzyme that in humans is encoded by the ATG4A gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodelling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. |
| Immunogen | Recombinant protein within Human ATG4A aa 87-314. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:1000: IHC:1:50-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AI627006 antibody
APG4 autophagy 4 homolog A antibody
Apg4a antibody ATG4 autophagy related 4 homolog A (S. cerevisiae) antibody ATG4 autophagy related 4 homolog A antibody ATG4A antibody ATG4A_HUMAN antibody Atg4al antibody AUT like 2 cysteine endopeptidase antibody AUT-like 2 cysteine endopeptidase antibody Autl2 antibody Autophagin 2 antibody Autophagin-2 antibody Autophagy related 4A cysteine peptidase antibody Autophagy related cysteine endopeptidase 2 antibody Autophagy related protein 4 homolog A antibody Autophagy-related cysteine endopeptidase 2 antibody Autophagy-related protein 4 homolog A antibody AV169859 antibody Cysteine protease ATG4A antibody hAPG4A antibody MGC107179 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of ATG4A on rat bone marrow. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-38, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Human placenta tissue, mouse testis tissue, rat bone marrow. |
|---|---|
| Application Notes | WB:1:500-1:1000: IHC:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
