Application
Fig1: Western blot analysis of Gephyrin on SK-Br-3 cell (1) and rat kidney tissue (2) lysate using anti-Gephyrin antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.| Product Name | Gephyrin [JG34-47] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Domain E antibody Domain G antibody GEPH antibody GEPH_HUMAN antibody GPH antibody GPHN antibody GPHRYN antibody KIAA1385 antibody Molybdopterin molybdenumtransferase antibody MPT adenylyltransferase antibody MPT Mo-transferase antibody |
| Product description | The sub-membraneous region at the postsynaptic membrane contains a number of proteins critical for receptor targeting. Gephyrin is a microtubule-associated protein highly expressed in brain and localized to neuronal postsynaptic membranes. Gephyrin is essential for the postsynaptic localization of the inhibitory glycine receptor and is thought to anchor the receptor to subsynaptic microtubules. The protein is expressed in most mammalian tissues with predominant expression in brain. At least five additional splice variants of Gephyrin ranging in molecular weight have been identified in rat and human brain tissue. |
| Immunogen | Recombinant protein within human Gephyrin aa 1-100. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Domain E antibody Domain G antibody GEPH antibody GEPH_HUMAN antibody GPH antibody GPHN antibody GPHRYN antibody KIAA1385 antibody Molybdopterin molybdenumtransferase antibody MPT adenylyltransferase antibody MPT Mo-transferase antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Plasma membrane. Cytoskeleton. |
Application
Fig1: Western blot analysis of Gephyrin on SK-Br-3 cell (1) and rat kidney tissue (2) lysate using anti-Gephyrin antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Gephyrin antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.| Positive Control | SK-Br-3, rat kidney tissue, human kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
