WB
Western blot analysis of Syntrophin alpha 1 on different lysates with Rabbit anti-Syntrophin alpha 1 antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Mouse heart tissue lysate, Lane 3: Rat skeletal muscle tissue lysate, Lane 4: Rat heart tissue lysate, Lane 5: Rat eyeball tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Syntrophin alpha 1 Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Syntrophins are cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. This gene is a member of the syntrophin gene family and encodes the most common syntrophin isoform found in cardiac tissues. The N-terminal PDZ domain of this syntrophin protein interacts with the C-terminus of the pore-forming alpha subunit (SCN5A) of the cardiac sodium channel Nav1.5. This protein also associates cardiac sodium channels with the nitric oxide synthase-PMCA4b (plasma membrane Ca-ATPase subtype 4b) complex in cardiomyocytes. This gene is a susceptibility locus for Long-QT syndrome (LQT) - an inherited disorder associated with sudden cardiac death from arrhythmia - and sudden infant death syndrome (SIDS). This protein also associates with dystrophin and dystrophin-related proteins at the neuromuscular junction and alters intracellular calcium ion levels in muscle tissue. |
| Immunogen | Synthetic peptide within mouse Syntrophin alpha 1 aa 174-223 / 503. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
Alpha-1-syntrophin antibody
Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody SNT1 antibody SNT2 antibody SNT3 antibody Snta1 antibody SNTA1_HUMAN antibody SNTB1 antibody SNTB2 antibody Syntrophin alpha 1 antibody Syntrophin beta 1 antibody Syntrophin beta 2 antibody Syntrophin-1 antibody TACIP1 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Sarcolemma, cytoskeleton, cell junction. |
WB
Western blot analysis of Syntrophin alpha 1 on different lysates with Rabbit anti-Syntrophin alpha 1 antibody at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate, Lane 2: Mouse heart tissue lysate, Lane 3: Rat skeletal muscle tissue lysate, Lane 4: Rat heart tissue lysate, Lane 5: Rat eyeball tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
