Application
Fig1: Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse skeletal muscle tissue lysate Lane 2: rat skeletal muscle tissue lysateApplication
Fig2: ICC staining of Syntrophin alpha 1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig5: Flow cytometric analysis of Syntrophin alpha 1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Syntrophin alpha 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 59 kDa dystrophin-associated protein A1 acidic component 1 antibody Alpha-1-syntrophin antibody Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody SNT1 antibody SNT2 antibody SNT3 antibody Snta1 antibody SNTA1_HUMAN antibody SNTB1 antibody SNTB2 antibody Syntrophin alpha 1 antibody Syntrophin beta 1 antibody Syntrophin beta 2 antibody Syntrophin-1 antibody TACIP1 antibody |
| Product description | Syntrophins are cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. This gene is a member of the syntrophin gene family and encodes the most common syntrophin isoform found in cardiac tissues. The N-terminal PDZ domain of this syntrophin protein interacts with the C-terminus of the pore-forming alpha subunit (SCN5A) of the cardiac sodium channel Nav1.5. This protein also associates cardiac sodium channels with the nitric oxide synthase-PMCA4b (plasma membrane Ca-ATPase subtype 4b) complex in cardiomyocytes. This gene is a susceptibility locus for Long-QT syndrome (LQT) - an inherited disorder associated with sudden cardiac death from arrhythmia - and sudden infant death syndrome (SIDS). This protein also associates with dystrophin and dystrophin-related proteins at the neuromuscular junction and alters intracellular calcium ion levels in muscle tissue. |
| Immunogen | Synthetic peptide within mouse Syntrophin aa 150-250. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC-PFC |
| WB:1:500-1:2,000: IHC-P:1:50-1:100: ICC:1:50-1:100: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 59 kDa dystrophin-associated protein A1 acidic component 1 antibody
Alpha-1-syntrophin antibody
Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody SNT1 antibody SNT2 antibody SNT3 antibody Snta1 antibody SNTA1_HUMAN antibody SNTB1 antibody SNTB2 antibody Syntrophin alpha 1 antibody Syntrophin beta 1 antibody Syntrophin beta 2 antibody Syntrophin-1 antibody TACIP1 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Sarcolemma, cytoskeleton, cell junction. |
Application
Fig1: Western blot analysis of Syntrophin alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse skeletal muscle tissue lysate Lane 2: rat skeletal muscle tissue lysate
Application
Fig2: ICC staining of Syntrophin alpha 1 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Flow cytometric analysis of Syntrophin alpha 1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-45, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, SW620, human kidney tissue, mouse skeletal muscle tissue, SiHa. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:100: ICC:1:50-1:100: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
