Application
Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysateApplication
Fig2: Western blot analysis of Dux on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig5: Flow cytometric analysis of Dux was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-52, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Dux |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Double homeobox 4 antibody Double homeobox protein 10 antibody Double homeobox protein 4 antibody Double homeobox protein 4/10 antibody DUX10 antibody DUX4L antibody DUX4L1 antibody |
| Product description | The double homeobox (DUX) proteins are encoded by 3.3-kilobase repeats found throughout the human genome. The DUX family includes DUX1, DUX2, DUX3, DUX4 and DUX5. Each of these family members, excluding DUX2, contains two homeobox domains. DUX2 contains only one homeobox domain. DUX1 and DUX5 are identical to one another and they share 98% identity with DUX3 and approximately 70% identity with DUX2. The genes encoding DUX5 and DUX3 both contain additional start sites that result in N-terminal extended isoforms. The homeodomains found in DUX5 and DUX1 are similar to those found in Pax-3, Pax-7, OTX1 and OTX2. DUX4, also known as DUX10, is capable of forming homodimers. In addition, the gene encoding DUX4 maps within the D4Z4 repeat unit that has been implicated in Facioscapulohumeral muscular dystrophy (FSHD). |
| Immunogen | Synthetic peptide within mouse Dux aa 200-500. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Double homeobox 4 antibody
Double homeobox protein 10 antibody Double homeobox protein 4 antibody Double homeobox protein 4/10 antibody DUX10 antibody DUX4L antibody DUX4L1 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (Observed band size) |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate
Application
Fig2: Western blot analysis of Dux on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dux antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Flow cytometric analysis of Dux was done on MG-63 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-52, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Hela cell lysate, Jurkat cell lysate, Raji cell lysates, rat testis tissue, mouse testis tissue, MG-63. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
