Application
Fig1: Western blot analysis of Syntrophin alpha 1 on SiHa cells lysates using anti-Syntrophin alpha 1 antibody at 1/1,000 dilution.Application
Fig2: ICC staining Syntrophin alpha 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Syntrophin alpha 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Syntrophin alpha 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Syntrophin alpha 1 [JG35-62] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 59 kDa dystrophin-associated protein A1 acidic component 1 antibody Acidic alpha 1 syntrophin antibody Alpha 1 syntrophin antibody Alpha-1-syntrophin antibody dJ1187J4.5 antibody Dystrophin associated protein A1 59kDa acidic component antibody LQT12 antibody OTTHUMP00000030650 antibody Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody SNT1 antibody Snta1 antibody SNTA1_HUMAN antibody Syntrophin 1 antibody Syntrophin-1 antibody TACIP1 antibody |
| Product description | The syntrophins are PDZ-domain-containing proteins that facilitate the recruitment of signaling proteins such as NOS1 to the dystrophin-associated protein complex. The syntrophin family are structurally related proteins that contain multiple protein interaction motifs. Syntrophins associate directly with dystrophin, the product of the Duchenne muscular dystrophy locus, and its homologs. α-Syntrophin has an important role in synapse formation and in the organization of utrophin, acetylcholine receptor and acetylcholinester-ase at the neuromuscular synapse. Specifically, NOS1 binds to α-Syntrophin at muscle sarcolemma. β2-Syntrophin is a modular adapter. In muscle cells, β2-Syntrophin interacts with members of the dystrophin family, which in-cludes utrophin. |
| Immunogen | Recombinant protein within human Syntrophin alpha 1 aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 59 kDa dystrophin-associated protein A1 acidic component 1 antibody Acidic alpha 1 syntrophin antibody Alpha 1 syntrophin antibody Alpha-1-syntrophin antibody dJ1187J4.5 antibody Dystrophin associated protein A1 59kDa acidic component antibody LQT12 antibody OTTHUMP00000030650 antibody Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody SNT1 antibody Snta1 antibody SNTA1_HUMAN antibody Syntrophin 1 antibody Syntrophin-1 antibody TACIP1 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cell junction. Plasma membrane. Cytoskeleton. |
| SwissProt ID | Q13424 |
|---|
Application
Fig1: Western blot analysis of Syntrophin alpha 1 on SiHa cells lysates using anti-Syntrophin alpha 1 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Syntrophin alpha 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Syntrophin alpha 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Syntrophin alpha 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HepG2, LOVO, SiHa, human colon tissue, human skeletal muscle tissue, mouse colon tissue, mouse skeletal muscle tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
