Application
Fig1: Western blot analysis of GABRD on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-54, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Flow cytometric analysis of GABRD was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | GABRD |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABA(A) receptor subunit delta antibody Gabrd antibody Gamma aminobutyric acid GABA A receptor delta antibody Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody Gamma-aminobutyric acid receptor subunit delta antibody GBRD_HUMAN antibody MGC45284 antibody |
| Product description | GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl- conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rα1-6, GABAA R β1-3, GABAA Rγ1-3, GABAA Rδ, GABAA Rε, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1α and GABAB R1β. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2, and -3). The GABA transporters function to terminate GABA action. |
| Immunogen | Synthetic peptide within rat GABRD aa 1-80. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:1,000-1:3,000: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | GABA(A) receptor subunit delta antibody
Gabrd antibody
Gamma aminobutyric acid GABA A receptor delta antibody Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody Gamma-aminobutyric acid receptor subunit delta antibody GBRD_HUMAN antibody MGC45284 antibody |
|---|---|
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Postsynaptic cell membrane, cell membrane. |
| SwissProt ID | O14764 |
|---|
Application
Fig1: Western blot analysis of GABRD on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-54, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Flow cytometric analysis of GABRD was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | HT-29 cell lysates, MCF-7. |
|---|---|
| Application Notes | WB:1:1,000-1:3,000: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
