Application
Fig1: Western blot analysis of hnRNP Q on K562 cell lysate using anti-hnRNP Q antibody at 1/1,000 dilution.Application
Fig2: ICC staining hnRNP Q in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining hnRNP Q in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.| Product Name | hnRNP Q [JG35-72] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cytoplasmic RNA-interacting protein antibody dJ3J17.2 antibody Glycine and tyrosine rich RNA binding protein antibody Glycine- and tyrosine-rich RNA-binding protein antibody GRY RBP antibody GRY-RBP antibody GRYRBP antibody Heterogeneous nuclear ribonucleoprotein Q antibody hnRNP Q antibody HNRPQ antibody HNRPQ_HUMAN antibody HNRPQ1 antibody NS1 associated protein 1 antibody NS1-associated protein 1 antibody NSAP1 antibody pp68 antibody RP1 3J17.2 antibody Synaptotagmin binding cytoplasmic RNA interacting protein antibody Synaptotagmin-binding antibody Syncrip antibody |
| Product description | Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) is involved in RNA processing and is necessary for efficient pre-mRNA splicing. hnRNP is widely expressed and developmentally regulated. hnRNP Q interacts with survival motor neuron protein (SMN). Loss of function of SMN results in spinal muscular atrophy, a common neurodegenerative disease. The most common deletion in SMN genes disrupts the interaction between SMN and hnRNP Q. hnRNP Q is upregulated after midnight, and this upregulation correlates with an abrupt decline in AANAT, the key enzyme in melatonin synthesis. Rhythmic AANAT mRNA degradation mediated in part by hnRNP Q implicates this enzyme in the regulation of circadian oscillation. |
| Immunogen | Recombinant protein to C-terminal human hnRNP Q. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | cytoplasmic RNA-interacting protein antibody dJ3J17.2 antibody Glycine and tyrosine rich RNA binding protein antibody Glycine- and tyrosine-rich RNA-binding protein antibody GRY RBP antibody GRY-RBP antibody GRYRBP antibody Heterogeneous nuclear ribonucleoprotein Q antibody hnRNP Q antibody HNRPQ antibody HNRPQ_HUMAN antibody HNRPQ1 antibody NS1 associated protein 1 antibody NS1-associated protein 1 antibody NSAP1 antibody pp68 antibody RP1 3J17.2 antibody Synaptotagmin binding cytoplasmic RNA interacting protein antibody Synaptotagmin-binding antibody Syncrip antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of hnRNP Q on K562 cell lysate using anti-hnRNP Q antibody at 1/1,000 dilution.
Application
Fig2: ICC staining hnRNP Q in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining hnRNP Q in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-hnRNP Q antibody. Counter stained with hematoxylin.| Positive Control | K562, LOVO, SiHa, rat testis tissue, human thyroid gland tissue, human breast tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
