Application
Fig1: Western blot analysis of Transferrin Receptor 2 on HepG2 cell using anti-Transferrin Receptor 2 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin Receptor 2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Transferrin Receptor 2 antibody. Counter stained with hematoxylin.| Product Name | Transferrin Receptor 2 [JG58-34] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HFE 3 antibody HFE3 antibody MGC126368 antibody TFR 2 antibody TfR2 antibody TFR2_HUMAN antibody TFRC 2 antibody TFRC2 antibody Transferrin receptor 2 antibody Transferrin receptor protein 2 antibody |
| Product description | Iron is a vital molecule for living organisms because it is involved in a wide variety of metabolic processes, such as oxygen transport, DNA synthesis and electron transport. Excessive iron uptake leads to tissue damage as a result of formation of free radicals. Iron uptake and storage is tightly regulated by the feedback system of iron responsive element-containing gene products and iron regulatory proteins that modulate the expression levels of the genes involved in iron metabolism. The transferrin receptor 2 (TFR2) mediates the uptake of transferrin-bound iron. It is involved in iron metabolism, hepatocyte function and erythrocyte differentiation, and is highly expressed as a protein in liver as well as in hepatocytes and erythroid precursors. The gene encoding human TRF2 maps to chromosome 7q22 and is expressed as an a isoform, which encodes a transmembrane protein, and a b isoform, which encodes a shorter, intracellular protein. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder that results in clinical complications, including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy and susceptibility to liver cancer. |
| Immunogen | Recombinant protein within human Transferrin Receptor 2 aa 100-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | HFE 3 antibody HFE3 antibody MGC126368 antibody TFR 2 antibody TfR2 antibody TFR2_HUMAN antibody TFRC 2 antibody TFRC2 antibody Transferrin receptor 2 antibody Transferrin receptor protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 89 kDa |
| Cellular Localization | Cell membrane. Cytoplasm. |
| SwissProt ID | Q9UP52 |
|---|
Application
Fig1: Western blot analysis of Transferrin Receptor 2 on HepG2 cell using anti-Transferrin Receptor 2 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin Receptor 2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Transferrin Receptor 2 antibody. Counter stained with hematoxylin.| Positive Control | HepG2, human liver tissue, human liver cancer tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
