Application
Fig1: Western blot analysis of ZHX2 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-64, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ZHX2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFP regulator 1 antibody AFR1 antibody Alpha fetoprotein regulator 1 antibody Alpha-fetoprotein regulator 1 antibody KIAA0854 antibody RAF antibody Regulator of AFP antibody Transcription factor ZHX2 antibody ZHX2 antibody ZHX2_HUMAN antibody Zinc finger and homeodomain protein 2 antibody Zinc fingers and homeoboxes 2 antibody Zinc fingers and homeoboxes protein 2 antibody |
| Product description | Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. Represses the promoter activity of the CDC25C gene stimulated by NFYA. May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity). |
| Immunogen | Recombinant protein within Human ZHX2 aa 538-733. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AFP regulator 1 antibody
AFR1 antibody
Alpha fetoprotein regulator 1 antibody Alpha-fetoprotein regulator 1 antibody KIAA0854 antibody RAF antibody Regulator of AFP antibody Transcription factor ZHX2 antibody ZHX2 antibody ZHX2_HUMAN antibody Zinc finger and homeodomain protein 2 antibody Zinc fingers and homeoboxes 2 antibody Zinc fingers and homeoboxes protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9Y6X8 |
|---|
Application
Fig1: Western blot analysis of ZHX2 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-64, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Positive Control | Daudi cell lysates, human esophagus tissue, mouse kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
