Application
Fig1: ICC staining KAT8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining KAT8 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining KAT8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue using anti-KAT8 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | KAT8 [JG36-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone acetyltransferase KAT8 antibody Histone acetyltransferase MYST1 antibody hMOF antibody K(lysine) acetyltransferase 8 antibody KAT 8 antibody Lysine acetyltransferase 8 antibody MOF antibody MOZ antibody MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 antibody MYST 1 antibody MYST histone acetyltransferase 1 antibody myst protein 1 antibody MYST-1 antibody MYST1 antibody MYST1_HUMAN antibody Ortholog of Drosophila males absent on the first (MOF) antibody Probable histone acetyltransferase MYST1 antibody SAS2 and TIP60 protein 1 antibody SAS2 antibody SAS3 antibody TIP60 protein 1 antibody YBF2 antibody YBF2/SAS3 antibody ZC2HC8 antibody |
| Product description | Dosage compensation ensures that males with a single X chromosome and females with two X chromosomes have the same amount of most X-linked gene products. In Drosophila, this is acheived by enhancing the level of transcription of the X chromosome in males. Proteins such as maleless, male specific lethal 1, 2 and 3, and males absent on the first (MOF) form a dosage compensation complex (DCC) that is required for the twofold increase of transcription of the male X chromosome. The DCC is preferentially associated with many sites on the X chromosome in somatic cells of males. The binding of the DCC to the X chromosome is dependent upon histone 4 acetylation at lysine 16, which is accomplished by MOF. In mammals, MOF (also designated hMOF, MYST1, or MOZ) belongs to the MYST family of histone acetyl transferases which are characterized by a unique C2HC-type zinc finger close to their HAT domains. MOF utilizes the zinc finger domain to contact the globular part of the nucleosome as well as the histone H4 N-terminal tail substrate. The carboxy terminal domain of human MOF also has histone acetyltransferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone acetyltransferases. |
| Immunogen | Recombinant protein within N-terminal human KAT8. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
|
| Species Reactivity | HumanRatMouse |
| Concentration | 1mg/ml |
| Alternative Names | Histone acetyltransferase KAT8 antibody
Histone acetyltransferase MYST1 antibody
hMOF antibody
K(lysine) acetyltransferase 8 antibody
KAT 8 antibody
Lysine acetyltransferase 8 antibody
MOF antibody
MOZ antibody
MOZ YBF2/SAS3 SAS2 and TIP60 protein 1 antibody MYST 1 antibody MYST histone acetyltransferase 1 antibody myst protein 1 antibody MYST-1 antibody MYST1 antibody MYST1_HUMAN antibody Ortholog of Drosophila males absent on the first (MOF) antibody Probable histone acetyltransferase MYST1 antibody SAS2 and TIP60 protein 1 antibody SAS2 antibody SAS3 antibody TIP60 protein 1 antibody YBF2 antibody YBF2/SAS3 antibody ZC2HC8 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Chromosome. Nucleus. |
| SwissProt ID | Q9H7Z6 |
|---|
Application
Fig1: ICC staining KAT8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining KAT8 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining KAT8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, SH-SY-5Y, SiHa, human cervix tissue, human cervix cancer tissue, rat cervix tissue, K562. |
|---|---|
| Application Notes | ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
