Application
Fig1: Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1,000 dilution.Application
Fig2: ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig5: Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CENPC [JG36-15] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENP-C 1 antibody CENP-C antibody CENPC_HUMAN antibody CENPC1 antibody Centromere autoantigen C antibody Centromere autoantigen C1 antibody Centromere protein C 1 antibody Centromere protein C antibody hcp-4 antibody ICEN7 antibody Interphase centromere complex protein 7 antibody MIF2 antibody |
| Product description | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle. |
| Immunogen | Recombinant protein within human CENPC aa 400-600. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CENP-C 1 antibody CENP-C antibody CENPC_HUMAN antibody CENPC1 antibody Centromere autoantigen C antibody Centromere autoantigen C1 antibody Centromere protein C 1 antibody Centromere protein C antibody hcp-4 antibody ICEN7 antibody Interphase centromere complex protein 7 antibody MIF2 antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q03188 |
|---|
Application
Fig1: Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig5: Flow cytometric analysis of K562 cells with CENPC antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | K562, Daudi, SH-SY-5Y, mouse spleen tissue, rat heart tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
