Application
Fig1: Western blot analysis of Fascin on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.Application
Fig6: Flow cytometric analysis of Fascin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Fascin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 55 kDa actin bundling protein antibody 55 kDa actin-bundling protein antibody Actin bundling protein antibody actin bundling protein, 55-KD antibody FAN 1 antibody FAN1 antibody Fascin 1 antibody Fascin actin bundling protein 1 antibody Fascin antibody Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody Fascin homolog 1 antibody Fascin, sea urchin, homolog of, 1 antibody Fascin1 antibody FLJ38511 antibody FSCN 1 antibody FSCN1 antibody FSCN1_HUMAN antibody HSN antibody p55 antibody Singed (Drosophila) like (sea urchin fascin homolog like) antibody Singed drosophila homolog like antibody Singed like (fascin homolog sea urchin) (Drosophila) antibody Singed like (fascin homolog sea urchin) antibody Singed like protein antibody Singed, drosophila, homolog of antibody Singed-like protein antibody SNL antibody Strongylocentrotus purpuratus antibody |
| Product description | Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF. Cell adhesion to extracellular matrix is an important physiological stimulus for organization of the Actin-based cytoskeleton. Adhesion to the matrix glycoprotein Thrombospondin 1 triggers the sustained formation of F-Actin microspikes that contain the Actin-bundling protein Fascin. These structures are also implicated in cell migration, which may be an important function of Thrombospondin 1 in tissue remodelling and wound repair. Fascin bundles Actin microfilaments within dynamic cellular structures such as microspikes, stress fibers and membrane ruffles. Fascin could serve as a prognostic factor for abnormal ovarian epithelial pathology and could be a novel target for the treatment of ovarian cancer. Fascin, an Actin-bundling protein, identifies dendritic cells in the blood and in tissues. |
| Immunogen | Recombinant protein within Human Fascin aa 1-120. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000: IHC:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 55 kDa actin bundling protein antibody
55 kDa actin-bundling protein antibody
Actin bundling protein antibody actin bundling protein 55-KD antibody FAN 1 antibody FAN1 antibody Fascin 1 antibody Fascin actin bundling protein 1 antibody Fascin antibody Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody Fascin homolog 1 antibody Fascin sea urchin homolog of 1 antibody Fascin1 antibody FLJ38511 antibody FSCN 1 antibody FSCN1 antibody FSCN1_HUMAN antibody HSN antibody p55 antibody Singed (Drosophila) like (sea urchin fascin homolog like) antibody Singed drosophila homolog like antibody Singed like (fascin homolog sea urchin) (Drosophila) antibody Singed like (fascin homolog sea urchin) antibody Singed like protein antibody Singed drosophila homolog of antibody Singed-like protein antibody SNL antibody Strongylocentrotus purpuratus antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cytoskeleton. Cytosol. |
Application
Fig1: Western blot analysis of Fascin on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Application
Fig6: Flow cytometric analysis of Fascin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000: IHC:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
