Application
Fig1: Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution. Positive control： Line 1: PC-3M Line 2: A549 Line 3: HepG2Application
Fig2: ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | IRAK2 [JG36-23] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | il1rak2 antibody Interleukin 1 receptor associated kinase 2 antibody Interleukin 1 receptor associated kinase like 2 antibody Interleukin-1 receptor-associated kinase-like 2 antibody IRAK 2 antibody IRAK-2 antibody Irak2 antibody IRAK2_HUMAN antibody MGC150550 antibody |
| Product description | IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. IRAK2 has been shown to interact with TRAF6 and Myd88. |
| Immunogen | Recombinant protein within human IRAK2 aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | il1rak2 antibody Interleukin 1 receptor associated kinase 2 antibody Interleukin 1 receptor associated kinase like 2 antibody Interleukin-1 receptor-associated kinase-like 2 antibody IRAK 2 antibody IRAK-2 antibody Irak2 antibody IRAK2_HUMAN antibody MGC150550 antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | O43187 |
|---|
Application
Fig1: Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution. Positive control： Line 1: PC-3M Line 2: A549 Line 3: HepG2
Application
Fig2: ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | PC-3M, A549, HepG2, Jurkat, human tonsil tissue, human prostate cancer tissue, human heart tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
