Application
Fig1: Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.| Product Name | Oct-1 [JG62-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ42836 antibody NF A1 antibody NF-A1 antibody Oct 1 antibody Oct 1B antibody Oct-1 antibody OCT1 antibody Octamer binding protein 1 antibody Octamer binding transcription factor 1 antibody Octamer-binding protein 1 antibody Octamer-binding transcription factor 1 antibody OTF 1 antibody OTF-1 antibody OTF1 antibody OTTHUMP00000032348 antibody OTTHUMP00000032350 antibody OTTHUMP00000032351 antibody PO21 antibody PO2F1 antibody PO2F1_HUMAN antibody POU class 2 homeobox 1 antibody POU domain class 2 transcription factor 1 antibody POU domain, class 2, transcription factor 1 antibody POU2F1 antibody |
| Product description | POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene. |
| Immunogen | Recombinant protein within human Oct-1 aa 1-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | FLJ42836 antibody
NF A1 antibody NF-A1 antibody Oct 1 antibody Oct 1B antibody Oct-1 antibody OCT1 antibody Octamer binding protein 1 antibody Octamer binding transcription factor 1 antibody Octamer-binding protein 1 antibody Octamer-binding transcription factor 1 antibody OTF 1 antibody OTF-1 antibody OTF1 antibody OTTHUMP00000032348 antibody OTTHUMP00000032350 antibody OTTHUMP00000032351 antibody PO21 antibody PO2F1 antibody PO2F1_HUMAN antibody POU class 2 homeobox 1 antibody POU domain class 2 transcription factor 1 antibody POU domain class 2 transcription factor 1 antibody POU2F1 antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 76 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Oct-1 on SH-SY-5Y cell (1) and A431 cell (2) lysate using anti-Oct-1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Oct-1 antibody. Counter stained with hematoxylin.| Positive Control | SH-SY-5Y, A431, rat large intestine tissue, human tonsil tissue, human colon cancer tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
