Application
Fig1: Sample: Eye (Mouse) Lysate at 40 ug Primary: Anti- MARCH3 (ER1903-02) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Product Name | MARCH3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase MARCH3 FLJ20096 MARCH-III March3 MARH3_HUMAN Membrane associated ring finger (C3HC4) 3 Membrane-associated RING finger protein 3 Membrane-associated RING-CH protein III RING finger protein 173 RNF173. |
| Product description | E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human MARCH3 101-200/253 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | E3 ubiquitin-protein ligase MARCH3 FLJ20096 MARCH-III March3 MARH3_HUMAN Membrane associated ring finger (C3HC4) 3 Membrane-associated RING finger protein 3 Membrane-associated RING-CH protein III RING finger protein 173 RNF173. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Cytoplasmic vesicle membrane. Early endosome membrane. |
| SwissProt ID | Q86UD3 |
|---|
Application
Fig1: Sample: Eye (Mouse) Lysate at 40 ug Primary: Anti- MARCH3 (ER1903-02) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
