Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (ER1903-03) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Sample: Siha(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (ER1903-03) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kDApplication
Fig3: Sample: Siha(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (ER1903-03) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (ER1903-03) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig5: Sample: Siha(human)cell Lysate at 30 ug MCF-7(human)cell Lysate at 30 ug U251(human)cell Lysate at 30 ug testis(rat) Lysate at 40 ug Primary: Anti-MARCH7 (ER1903-03) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 73 kD| Product Name | MARCH7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AXO AXOT Axotrophin E3 ubiquitin-protein ligase MARCH7 MARCH VII MARCH-VII March7 MARH7_HUMAN Membrane associated RING CH protein VII Membrane associated ring finger (C3HC4) 7 E3 ubiquitin protein ligase membrane associated ring finger C3HC4 7 Membrane associated RING finger protein 7 Membrane-associated RING finger protein 7 Membrane-associated RING-CH protein VII RING finger protein 177 RNF177. |
| Product description | E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Immunogen | KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin 31-130/704 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | AXO AXOT Axotrophin E3 ubiquitin-protein ligase MARCH7 MARCH VII MARCH-VII March7 MARH7_HUMAN Membrane associated RING CH protein VII Membrane associated ring finger (C3HC4) 7 E3 ubiquitin protein ligase membrane associated ring finger C3HC4 7 Membrane associated RING finger protein 7 Membrane-associated RING finger protein 7 Membrane-associated RING-CH protein VII RING finger protein 177 RNF177. |
|---|---|
| Molecular Weight(MW) | 78 KDa |
| SwissProt ID | Q9H992 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (ER1903-03) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Sample: Siha(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (ER1903-03) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD
Application
Fig3: Sample: Siha(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (ER1903-03) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 73 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (ER1903-03) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig5: Sample: Siha(human)cell Lysate at 30 ug MCF-7(human)cell Lysate at 30 ug U251(human)cell Lysate at 30 ug testis(rat) Lysate at 40 ug Primary: Anti-MARCH7 (ER1903-03) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78kD Observed band size: 73 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
