Application
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).Application
Fig2: Sample: MCF-7 (human)cell Lysate at 40 ug Primary: Anti- MARCH9(ER1903-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38 kD| Product Name | MARCH9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin protein ligase MARCH9 MARCH IX Membrane associated RING CH protein IX membrane associated ring finger (C3HC4) 9 Membrane associated RING finger protein 9 RING finger protein 179 RNF179 MARH9_HUMAN. |
| Product description | E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Immunogen | KLH conjugated synthetic peptide derived from human MARCH9 201-300/346 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | E3 ubiquitin protein ligase MARCH9 MARCH IX Membrane associated RING CH protein IX membrane associated ring finger (C3HC4) 9 Membrane associated RING finger protein 9 RING finger protein 179 RNF179 MARH9_HUMAN. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. |
| SwissProt ID | Q86YJ5 |
|---|
Application
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
Application
Fig2: Sample: MCF-7 (human)cell Lysate at 40 ug Primary: Anti- MARCH9(ER1903-04)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38 kD| Positive Control | Ubiquitously expressed. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
