Application
Fig1: Protein: HepG2 lyates(hu); Primary: Rabbit Anti- 5HT1A Receptor (ER1903-07) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kD Observed band size:56 kDApplication
Fig2: Protein: Brain(Mouse) Lysate at 40 ug Primary: Rabbit Anti- 5HT1A Receptor (ER1903-07) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kD Observed band size:56 kDApplication
Fig3: Sample: Jurkat(Human) Cell Lysate at 40 ug Primary: Anti-5HT1A Receptor (ER1903-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Product Name | 5HT1A Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5HT1A Receptor HTR1A 5-HTR1A 5 HT 1A 5 HT receptor 1A 5 HT1A 5 HT1A receptor 5 hydroxytryptamine (serotonin) receptor 1A 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled 5 hydroxytryptamine receptor 1A 5-HT-1A 5-HT1A 5-hydroxytryptamine receptor 1A 5HT 1A 5HT 1A receptor 5HT receptor 1A 5HT1a 5HT1A_HUMAN ADRB2RL1 ADRBRL1 G 21 G protein coupled receptor G-21 Gpcr 18 Gpcr18 Guanine nucleotide binding regulatory protein-coupled receptor PFMCD Serotonin 1A receptor Serotonin 5HT1A receptor Serotonin receptor 1A |
| Product description | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5-HTR1A 301-400/422 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 5HT1A Receptor HTR1A 5-HTR1A 5 HT 1A 5 HT receptor 1A 5 HT1A 5 HT1A receptor 5 hydroxytryptamine (serotonin) receptor 1A 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled 5 hydroxytryptamine receptor 1A 5-HT-1A 5-HT1A 5-hydroxytryptamine receptor 1A 5HT 1A 5HT 1A receptor 5HT receptor 1A 5HT1a 5HT1A_HUMAN ADRB2RL1 ADRBRL1 G 21 G protein coupled receptor G-21 Gpcr 18 Gpcr18 Guanine nucleotide binding regulatory protein-coupled receptor PFMCD Serotonin 1A receptor Serotonin 5HT1A receptor Serotonin receptor 1A |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P19327 |
|---|
Application
Fig1: Protein: HepG2 lyates(hu); Primary: Rabbit Anti- 5HT1A Receptor (ER1903-07) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kD Observed band size:56 kD
Application
Fig2: Protein: Brain(Mouse) Lysate at 40 ug Primary: Rabbit Anti- 5HT1A Receptor (ER1903-07) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:46 kD Observed band size:56 kD
Application
Fig3: Sample: Jurkat(Human) Cell Lysate at 40 ug Primary: Anti-5HT1A Receptor (ER1903-07) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD| Positive Control | Rat brain, HepG2 cell, Mouse Brain Lysate, Jurkat Cell Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
