Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT1F Receptor) Polyclonal Antibody, Unconjugated (ER1903-08) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti- 5HT1F Receptor (ER1903-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kDApplication
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-5HT1F Polyclonal Antibody, Unconjugated(ER1903-08) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT1F Receptor) Polyclonal Antibody, Unconjugated (ER1903-08) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | 5HT1F Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SR-1F 5 HT 1F 5 HT1F 5 hydroxytryptamine (serotonin) receptor 1F 5 hydroxytryptamine 1F receptor 5 hydroxytryptamine receptor 1E beta 5 hydroxytryptamine receptor 1F 5 hydroxytryptamine serotonin receptor 1E beta 5 hydroxytryptamine serotonin receptor 1F 5HT 1F 5HT1F HTR 1F HTR1EB HTR1EL HTR1F MR 77 MR77 Serotonin receptor 1E beta Serotonin receptor gene 5HT1F_HUMAN. |
| Product description | 5HT1F is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. 5HT1F is a member of the GPCR family (subfamily: Serotonin). Expression of 5HT1F has been reported in various brain structures, including cortex, hippocampus, trigeminal ganglia, and cerebral blood vessels. Expression in peripheral tissues is limited to uterus, mesentery, and artery. No expression has been detected in heart, kidney, liver, pancreas, or spleen. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5HT1F Receptor/SR-1F 1-100/366 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | SR-1F 5 HT 1F 5 HT1F 5 hydroxytryptamine (serotonin) receptor 1F 5 hydroxytryptamine 1F receptor 5 hydroxytryptamine receptor 1E beta 5 hydroxytryptamine receptor 1F 5 hydroxytryptamine serotonin receptor 1E beta 5 hydroxytryptamine serotonin receptor 1F 5HT 1F 5HT1F HTR 1F HTR1EB HTR1EL HTR1F MR 77 MR77 Serotonin receptor 1E beta Serotonin receptor gene 5HT1F_HUMAN. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Cell membrane; Multi pass membrane protein. |
| SwissProt ID | P30939 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT1F Receptor) Polyclonal Antibody, Unconjugated (ER1903-08) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti- 5HT1F Receptor (ER1903-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD
Application
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-5HT1F Polyclonal Antibody, Unconjugated(ER1903-08) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT1F Receptor) Polyclonal Antibody, Unconjugated (ER1903-08) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
