Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug BV2(Mouse) Cell Lysate at 30 ug Primary: Anti-5HT2A Receptor (ER1903-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kDApplication
Fig2: Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (ER1903-09) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | 5HT2A Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5-hydroxytryptamine receptor 2A 5-hydroxytryptamine receptor Serotonin receptor 2A 5-HT-2A 5-HT-2 HTR2A Htr2a 5 HT 2 5HTR2A 5 HT 2A 5 HT2 receptor 5 HT2A 5 hydroxytryptamine receptor 2A 5-HT-2 5-HT-2A 5-hydroxytryptamine 2A receptor 5-hydroxytryptamine receptor 2A 5HT2A_HUMAN HTR 2 HTR 2A HTR2 HTR2A. |
| Product description | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5HT2A Receptor 65-160/471 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: IF:1:200-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 5-hydroxytryptamine receptor 2A 5-hydroxytryptamine receptor Serotonin receptor 2A 5-HT-2A 5-HT-2 HTR2A Htr2a 5 HT 2 5HTR2A 5 HT 2A 5 HT2 receptor 5 HT2A 5 hydroxytryptamine receptor 2A 5-HT-2 5-HT-2A 5-hydroxytryptamine 2A receptor 5-hydroxytryptamine receptor 2A 5HT2A_HUMAN HTR 2 HTR 2A HTR2 HTR2A. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses. |
| SwissProt ID | P28223 |
|---|
Application
Fig1: Sample: U251(Human) Cell Lysate at 30 ug BV2(Mouse) Cell Lysate at 30 ug Primary: Anti-5HT2A Receptor (ER1903-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD
Application
Fig2: Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (ER1903-09) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (ER1903-09) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | U251(Human) Cell Lysate, U-2OS, Mouse brain, Rat brain, rat stomach tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/Test: IF:1:200-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
