Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti- 5HT2A Receptor (ER1903-10)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 54/59kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated (ER1903-10) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | 5HT2B Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SR-2B 5 HT 2B 5 HT 2B receptor 5 HT(2B) 5 HT2B 5 hydroxytryptamine (serotonin) receptor 2B 5 hydroxytryptamine (serotonin) receptor 2B G protein coupled 5 hydroxytryptamine 2B receptor 5 hydroxytryptamine receptor 2B 5-HT-2B 5-HT2B 5-hydroxytryptamine receptor 2B 5HT(2B) 5HT2B_HUMAN 5HT2F Htr 2b Htr2b NP75 protein SEROTONIN 5HT2B RECEPTOR Serotonin receptor 2B Stomach fundus serotonin receptor. |
| Product description | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5HT2B Receptor/SR-2B 1-100/481 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SR-2B 5 HT 2B 5 HT 2B receptor 5 HT(2B) 5 HT2B 5 hydroxytryptamine (serotonin) receptor 2B 5 hydroxytryptamine (serotonin) receptor 2B G protein coupled 5 hydroxytryptamine 2B receptor 5 hydroxytryptamine receptor 2B 5-HT-2B 5-HT2B 5-hydroxytryptamine receptor 2B 5HT(2B) 5HT2B_HUMAN 5HT2F Htr 2b Htr2b NP75 protein SEROTONIN 5HT2B RECEPTOR Serotonin receptor 2B Stomach fundus serotonin receptor. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P41595 |
|---|
Application
Fig1: Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti- 5HT2A Receptor (ER1903-10)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 54/59kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated (ER1903-10) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in brain. Higher levels of expression are observed in the cerebellum, occipital cortex and frontal cortex. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
