Application
Fig1: Western blot analysis of FHL2 on different tissue lysates using anti-FHL2 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat heart Lane 2: Mouse heartApplication
Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.| Product Name | FHL2 [JG36-42] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AAG 11 antibody AAG11 antibody Aging associated gene 11 antibody Down regulated in rhabdomyosarcoma LIM protein antibody Downregulated in rhabdomyosarcoma LIM protein antibody DRAL antibody FHL 2 antibody FHL-2 antibody Fhl2 antibody FHL2 protein antibody FHL2_HUMAN antibody Four and a half LIM domain protein 2 antibody Four and a half LIM domains 2 antibody Four and a half LIM domains protein 2 antibody KIAA0990 antibody LIM domain protein DRAL antibody Skeletal muscle LIM protein 3 antibody Skeletal muscle LIM-protein 3 antibody SLIM 3 antibody SLIM-3 antibody SLIM3 antibody |
| Product description | The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group. |
| Immunogen | Recombinant protein within C-terminal human FHL2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AAG 11 antibody AAG11 antibody Aging associated gene 11 antibody Down regulated in rhabdomyosarcoma LIM protein antibody Downregulated in rhabdomyosarcoma LIM protein antibody DRAL antibody FHL 2 antibody FHL-2 antibody Fhl2 antibody FHL2 protein antibody FHL2_HUMAN antibody Four and a half LIM domain protein 2 antibody Four and a half LIM domains 2 antibody Four and a half LIM domains protein 2 antibody KIAA0990 antibody LIM domain protein DRAL antibody Skeletal muscle LIM protein 3 antibody Skeletal muscle LIM-protein 3 antibody SLIM 3 antibody SLIM-3 antibody SLIM3 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of FHL2 on different tissue lysates using anti-FHL2 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat heart Lane 2: Mouse heart
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FHL2 antibody. Counter stained with hematoxylin.| Positive Control | Rat heart tissue, mouse heart tissue, human fetal heart tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
