Application
Fig1: Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody (ER1903-11-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (ER1903-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (ER1903-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kDApplication
Fig4: Sample: Lovo Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (ER1903-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD| Product Name | 5HT3B receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 HT3 B 5 HT3B 5 HTR3B 5 hydroxytryptamine 3 receptor B subunit 5 hydroxytryptamine receptor 3B 5 hydroxytryptamine serotonin receptor 3B 5-HT3-B 5-HT3B 5-HTR3B 5-hydroxytryptamine 3 receptor B subunit 5-hydroxytryptamine receptor 3B 5-hydroxytryptamine serotonin receptor 3B 5HT3B_HUMAN 5HTR3B HTR3B Serotonin gated ion channel subunit serotonin receptor 3B. |
| Product description | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor 101-200/441 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 5 HT3 B 5 HT3B 5 HTR3B 5 hydroxytryptamine 3 receptor B subunit 5 hydroxytryptamine receptor 3B 5 hydroxytryptamine serotonin receptor 3B 5-HT3-B 5-HT3B 5-HTR3B 5-hydroxytryptamine 3 receptor B subunit 5-hydroxytryptamine receptor 3B 5-hydroxytryptamine serotonin receptor 3B 5HT3B_HUMAN 5HTR3B HTR3B Serotonin gated ion channel subunit serotonin receptor 3B. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A. |
| SwissProt ID | O95264 |
|---|
Application
Fig1: Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibody (ER1903-11-AF488), Dilution: 1ug in 100 ul 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugated (ER1903-11) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (ER1903-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD
Application
Fig4: Sample: Lovo Cell (Human) Lysate at 40 ug Primary: Anti- 5HT3B receptor (ER1903-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 55 kD| Positive Control | Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
