Application
Fig1: Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-5HT4 Receptor (ER1903-12) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Product Name | 5HT4 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 HT 4 5 HT4 5 HT4R 5 hydroxytryptamine 4 receptor 5 hydroxytryptamine receptor 4 5 hydroxytryptamine serotonin receptor 4 5 hydroxytryptamine4 receptor 5-HT-4 5-ht4 5-HT4R 5H4 5HT 4 5HT4 5ht4 receptor 5HT4R Cardiac 5 HT4 receptor HTR 4 HTR4 serotonin 4 receptor Serotonin 5 HT4 receptor serotonin 5-ht-4 receptor serotonin 5-ht-4a. Serotonin receptor 4 5HT4R_HUMAN. |
| Product description | The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5HT4 Receptor 161-270/388 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | 5 HT 4 5 HT4 5 HT4R 5 hydroxytryptamine 4 receptor 5 hydroxytryptamine receptor 4 5 hydroxytryptamine serotonin receptor 4 5 hydroxytryptamine4 receptor 5-HT-4 5-ht4 5-HT4R 5H4 5HT 4 5HT4 5ht4 receptor 5HT4R Cardiac 5 HT4 receptor HTR 4 HTR4 serotonin 4 receptor Serotonin 5 HT4 receptor serotonin 5-ht-4 receptor serotonin 5-ht-4a. Serotonin receptor 4 5HT4R_HUMAN. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. |
| SwissProt ID | Q13639 |
|---|
Application
Fig1: Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-5HT4 Receptor (ER1903-12) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Positive Control | Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
