Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (ER1903-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (bs- 2127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kDApplication
Fig3: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (ER1903-13) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (ER1903-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | 5HT4 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5-HTR4 (5-hydroxytryptamine (serotonin) receptor 4a) Htr4a 5-HT4 5-HT4A 5HT4 Htr45 HT 4 5 HT4 5 HT4R 5 hydroxytryptamine 4 receptor 5 hydroxytryptamine receptor 4 5 hydroxytryptamine serotonin receptor 4 5 hydroxytryptamine4 receptor 5HT4 5HT4R Cardiac 5 HT4 receptor HTR 4 HTR4 Serotonin 5 HT4 receptor Serotonin receptor 4 5HT4R_HUMAN. |
| Product description | This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. |
| Immunogen | KLH conjugated synthetic peptide derived from human 5-HTR4 21-120/388 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
|
| Species Reactivity | HumanMouseRatChickenCow |
| Concentration | 1mg/ml |
| Alternative Names | 5-HTR4 (5-hydroxytryptamine (serotonin) receptor 4a) Htr4a 5-HT4 5-HT4A 5HT4 Htr45 HT 4 5 HT4 5 HT4R 5 hydroxytryptamine 4 receptor 5 hydroxytryptamine receptor 4 5 hydroxytryptamine serotonin receptor 4 5 hydroxytryptamine4 receptor 5HT4 5HT4R Cardiac 5 HT4 receptor HTR 4 HTR4 Serotonin 5 HT4 receptor Serotonin receptor 4 5HT4R_HUMAN. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. |
| SwissProt ID | Q13639 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (ER1903-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (bs- 2127R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD
Application
Fig3: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-5HT4 Receptor (ER1903-13) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (ER1903-13) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
