Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AARE) Polyclonal Antibody, Unconjugated (ER1903-16) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- AARE (ER1903-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 78 kDApplication
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- AARE (ER1903-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 78 kD| Product Name | AARE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AARE ACPH Acyl peptide hydrolase Acylamino acid releasing enzyme Acylaminoacyl peptidase APEH APH D3F15S2 D3S48E DNF15S2 N acylaminoacyl peptide hydrolase OPH Oxidized protein hydrolase ACPH_HUMAN. |
| Product description | This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. |
| Immunogen | KLH conjugated synthetic peptide derived from human AARE 101-200/732 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | AARE ACPH Acyl peptide hydrolase Acylamino acid releasing enzyme Acylaminoacyl peptidase APEH APH D3F15S2 D3S48E DNF15S2 N acylaminoacyl peptide hydrolase OPH Oxidized protein hydrolase ACPH_HUMAN. |
|---|---|
| Molecular Weight(MW) | 81 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P13798 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AARE) Polyclonal Antibody, Unconjugated (ER1903-16) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug U2OS(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- AARE (ER1903-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 78 kD
Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- AARE (ER1903-16) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 78 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
