Application
Fig1: Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.Application
Fig2: ICC staining GEF H1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GEF H1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | GEF H1 [JG36-46] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AA408978 antibody ARHG2 antibody ARHG2_HUMAN antibody ARHGEF 2 antibody ARHGEF-2 antibody ARHGEF2 antibody GEF antibody GEF H1 antibody GEF-H1 antibody GEFH1 antibody Guanine nucleotide exchange factor H1 antibody KIAA0651 antibody Lbcl1 antibody Lfc antibody LFP40 antibody MGC95068 antibody Microtubule-regulated Rho-GEF antibody mKIAA0651 antibody P40 antibody Proliferating cell nucleolar antigen p40 antibody Protein GEF-H1 antibody Rho guanine nucleotide exchange factor 2 antibody rho/rac guanine nucleotide exchange factor (GEF) 2 antibody rho/rac guanine nucleotide exchange factor 2 antibody rho/rac guanine nucleotide exchange factor antibody |
| Product description | Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. |
| Immunogen | Recombinant protein corresponding to C-terminal human GEF H1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AA408978 antibody ARHG2 antibody ARHG2_HUMAN antibody ARHGEF 2 antibody ARHGEF-2 antibody ARHGEF2 antibody GEF antibody GEF H1 antibody GEF-H1 antibody GEFH1 antibody Guanine nucleotide exchange factor H1 antibody KIAA0651 antibody Lbcl1 antibody Lfc antibody LFP40 antibody MGC95068 antibody Microtubule-regulated Rho-GEF antibody mKIAA0651 antibody P40 antibody Proliferating cell nucleolar antigen p40 antibody Protein GEF-H1 antibody Rho guanine nucleotide exchange factor 2 antibody rho/rac guanine nucleotide exchange factor (GEF) 2 antibody rho/rac guanine nucleotide exchange factor 2 antibody rho/rac guanine nucleotide exchange factor antibody |
|---|---|
| Molecular Weight(MW) | 111 kDa |
| Cellular Localization | Cytoskeleton. Golgi apparatus. Plasma membrane. |
Application
Fig1: Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.
Application
Fig2: ICC staining GEF H1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GEF H1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293T, A431, HUVEC, SH-SY-5Y, rat brain tissue, human tonsil tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
