Application
Fig1: Sample: Lane1: Testis (Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-ABP/SHBG(ER1903-18) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 44kDApplication
ng buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | ABP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Androgen binding protein MGC126834 MGC138391 SBP Sex steroid binding protein SHBG TEBG Testis specific androgen binding protein Testosterone binding beta globulin Testosterone Estradiol Binding Globulin Testosterone estrogen binding globulin UniProtKB/Swiss-Prot: P04278.2 SHBG_HUMAN. |
| Product description | Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. |
| Immunogen | KLH conjugated synthetic peptide derived from human ABP 51-150/402 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Androgen binding protein MGC126834 MGC138391 SBP Sex steroid binding protein SHBG TEBG Testis specific androgen binding protein Testosterone binding beta globulin Testosterone Estradiol Binding Globulin Testosterone estrogen binding globulin UniProtKB/Swiss-Prot: P04278.2 SHBG_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Secreted (By similarity). Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis (By similarity). |
| SwissProt ID | P04278 |
|---|
Application
Fig1: Sample: Lane1: Testis (Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-ABP/SHBG(ER1903-18) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kD Observed band size : 44kD
Application
ng buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (ER1903-18) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Isoform 1 and isoform 2 are present in liver and testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
